Mouse monoclonal antibody to rat p75NTR [MC192] - FITCM-016-100-FT
Catalog No#: M-016-100-FT
Product Name: Mouse monoclonal antibody to rat p75NTR [MC192] - FITC
Alternative Name: Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Product Type: Monoclonal Antibody
Description: Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9.
Size: 100 ug
Applications: Not Suitable for WB; IHC, Frozen; IF, Frozen; IC; FC;
Recommended Dilutions: The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells.Immunofluorescence (1-2 ug/mL), flow cytometry (20 ug/mL) NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. (10-20ug/mL). ELISA (1 Suggested working dilutions: The antibody is not appropriate for Western Blots. Antibody will not work in traditional formalin fixed tissues. The recommended concentration for FACS is 20 µg/mL and at least 1 in 5000 dilution is recommended for 1 site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Clone Name: MC192
Immunogen: Rat p75NTR
Purity: Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Species Reactivity: Reacts with Rat. Does not react with Mouse or Human p75 NGFR
Specification: MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. | Reacts with rat. Does not react with mouse or human p75 NGFR
Shipping Conditions: 2-8°C (on cold pack)
Storage Conditions: The antibody conjugate can be stored at 4 °C for up to 4 months with the addition of appropriate antibacterial agent.
Expiration Date: Four months after purchase.
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=265
Product Link: http://www.biosensis.com/product_info.php/mouse-monoclonal-antibody-p75ntr-mc192-fitc-p-265