Sheep antibody to c-FOS (2-17): affinity purified

Supplier: Biosensis Catalog No#: S-045-50 Product Name: Sheep antibody to c-FOS (2-17): affinity purified Alternative Name: Proto-oncogene protein cFOS; c-FOS Product Type: Polyclonal Antibody Description: FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain Size: 50 ug Host: Sheep Isotype: Applications: WB; IHC, Frozen; Recommended Dilutions: IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting. Clone Name: Conjugation: Formation: Lyophilized Immunogen: A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat. Purity: Affinity purified Reconstitution: Reconstitute in 50 µL of sterile water. Centrifuge to remove any insoluble material. Species Reactivity: This antiserum is known to react with Rat, rabbit and hamster cFOS. Specification: This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. | This antiserum is known to react with rat, rabbit and hamster cFOS. Shipping Conditions: 25°C (Ambient) Storage Conditions: Store lyophilized product at 2-8C. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Expiration Date: 12 months after purchase Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=155 Product Link: http://www.biosensis.com/product_info.php/sheep-antibody-affinity-purified-p-155