Sheep antibody to beta NGF: affinity purifiedS-051-50
Catalog No#: S-051-50
Product Name: Sheep antibody to beta NGF: affinity purified
Alternative Name: Beta-nerve growth factor
Product Type: Polyclonal Antibody
Description: FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Size: 50 ug
Applications: WB; IHC, Frozen; ELISA; Neut, Neutralizing
Recommended Dilutions: IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen: Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Purity: Affinity purified antibody
Reconstitution: Reconstitute in 50 µL of sterile water. Centrifuge to remove any insoluble material.
Species Reactivity: This antiserum is known to cross react with Mouse, Rat, Human and avian NGF but not bovine NGF.
Specification: A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. | This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Shipping Conditions: 25°C (Ambient)
Storage Conditions: After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiration Date: 12 months after purchase
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=163
Product Link: http://www.biosensis.com/product_info.php/sheep-antibody-beta-affinity-purified-p-163