Sheep antibody to Connexin-45 (354-367): whole serumS-061-100
Catalog No#: S-061-100
Product Name: Sheep antibody to Connexin-45 (354-367): whole serum
Alternative Name: Gap junction gamma-1 protein; Gap junction alpha-7 protein; Cx45; GJC1; GJA7
Product Type: Polyclonal Antibody
Description: Connexin-45 is a component of gap junctions, which are composed of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily. Alternatively spliced isoforms have been described.
Size: 100 uL
Applications: WB; IF, Frozen;
Recommended Dilutions: Immunohistochemistry, Western blot. A dilution of 1:10000 to 1: 20000 is recommended for IHC and 1:20000 to 1:50000 for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen: A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa: 354-367) conjugated to diphtheria toxoid has been used as the immunogen.
Purity: Whole serum
Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Species Reactivity: This antiserum reacts to human Connexin-45.
Specification: Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47 kDa which is abolished by preincubation with the immunising peptide. No such band is detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40. | This antiserum reacts to human Connexin-45.
Shipping Conditions: 25°C (Ambient)
Storage Conditions: After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiration Date: 12 months after purchase
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=186
Product Link: http://www.biosensis.com/product_info.php/sheep-antibody-connexin-whole-serum-p-186