Sheep antibody to Connexin-37 (265-280): whole serumS-062-100
Catalog No#: S-062-100
Product Name: Sheep antibody to Connexin-37 (265-280): whole serum
Alternative Name: Gap junction alpha-4 protein; Cx37; Gja4; Cxn-37
Product Type: Polyclonal Antibody
Description: FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Connexin-37 may also mediate hyperexcitability followin nerve injury. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.
Size: 100 uL
Applications: WB; IF, Frozen;
Recommended Dilutions: Immunohistochemistry, Western blot. A dilution of 1:1000 to 1: 5000 is recommended for IHC and 1:5000 to 1:10000 for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen: A synthetic peptide (YLPMGE GPSSPPCPTY) as part of rat Connexin-37 protein (aa: 265-280) conjugated to diphtheria toxoid
Purity: Whole serum
Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Species Reactivity: This antiserum recognises Rat and Mouse Connexin-37 protein.
Specification: Western blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-37 shows two bands of molecular weight 36 and 37 kDa which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-40, Connexin-43 or Connexin-45. | This antiserum recognises rat and mouse Connexin-37 protein.
Shipping Conditions: 25°C (Ambient)
Storage Conditions: After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiration Date: 12 months after purchase
Datasheet Link: http://www.biosensis.com/pdf_datasheet.php?products_id=185
Product Link: http://www.biosensis.com/product_info.php/sheep-antibody-connexin-whole-serum-p-185