Rapid Human Monoclonal Antibody Isotyping Kit-1 (10 tests)

Supplier: Antagen Pharmaceuticals, Inc.

Catalog#: ISO-Hu-10

Product Type: Assay Kit

Product Category: Isotyping Assay Kit

Size: 10 Assays

Descriptions: This rapid human antibody isotyping kit detects 7 human antibody heavy chain isotypes and 2 light chain subclasses (IgG1, IgG2a, IgG3, IgG4, IgA1, IgA2, IgM, kappa, and lambda) in five minutes, faster and more convenient than ELISA. This kit is shaped as a cassette. After loading 100 µL of solution, a dark line will appear showing the isotype of the antibody. Another line will appear on the control (C), showing a successful test.

Applications: The Rapid Human Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class (7) and subclass (2) determination. It can determine isotypes and subclass of your human antibody  among IgG1, IgG2a, IgG3, IgG4, IgA1, IgA2, IgM, kappa, and lambda.

Kit Components: The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2, IgG3 and IgG4, and the other cassette is for determining IgM, IgA1, IgA2, κ and λ. Both strips contain a control line “C”.

Sample Type and Dilution Conditions:

Acceptable sample types include hybridoma culture supernatant and purified monoclonal antibody. This kit can also be used to determine the relative distribution and changes of the isotypes in serum under health and disease status.

Cell Culture Supernatant: Depending on the actual titre of the Ab-producing cells or hybridomas, recommended dilution is 1:2~1:50 in PBS.

Purified Antibody: Dilute samples to 0.5 – 2.0 μg/ml in PBS.

Serum: Serum can be diluted 100-500 fold in PBS.

Storage Conditions: Room Temperature

Shipping Conditions: Cassettes are individually packaged and shipped at ambient temperature.

Shelf Life: 18 Months