Dog Interleukin 1 Beta (IL1b) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Interleukin 1 Beta Alternative Names: IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Cytokine;Tumor immunity;Infection immunity; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Canis familiaris; Canine (Dog) Detection Sensitivity: < 3.1pg/mL Detection Range: 7.8-500pg/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA563Ca.html