Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Anterior Gradient Protein 2
Alternative Names: GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2 (AGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anterior Gradient Protein 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient Protein 2 (AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anterior Gradient Protein 2 (AGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Tumor immunity;Reproductive science;Bone metabolism;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Organism Species: Homo sapiens (Human)
Detection Sensitivity: < 6.4pg/mL
Detection Range: 15.6-1000pg/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SEC285Hu.html