Human Claudin 3 (CLDN3) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Claudin 3 Alternative Names: C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 3 (CLDN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 3 (CLDN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 3 (CLDN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 3 (CLDN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Signal transduction; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma, tissue homogenates and other biological fluids Organism Species: Homo sapiens (Human) Detection Sensitivity: < 0.112ng/mL Detection Range: 0.312-20ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEF293Hu.html