Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: C1q And Tumor Necrosis Factor Related Protein 9
Alternative Names: CTRP9; C1QTNF9A; AQL1; Complement C1q and tumor necrosis factor-related protein 9A
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Tumor immunity;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: serum, plasma and other biological fluids
Organism Species: Homo sapiens (Human)
Detection Sensitivity: < 6.3pg/mL
Detection Range: 15.6-1,000pg/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SER877Hu.html