Human Angiopoietin Like Protein 8 (ANGPTL8) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Angiopoietin Like Protein 8 Alternative Names: RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Developmental science; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Homo sapiens (Human) Detection Sensitivity: < 0.058ng/mL Detection Range: 0.156-10ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEW803Hu.html