Rabbit Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Intercellular Adhesion Molecule 1 Alternative Names: CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 1 (ICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 1 (ICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Oryctolagus cuniculus (Rabbit) Detection Sensitivity: < 2.53pg/mL Detection Range: 6.25-400pg/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA548Rb.html