Recombinant Mouse HVEM / TNFRSF14 Protein (His & Fc tag)

Supplier: Elabscience Product Name: Recombinant Mouse HVEM / TNFRSF14 Protein (His & Fc tag) Catalog No. PKSM040930 Product Type: recombinant protein Size: 200 ug Activity: Measured by its binding ability in a functional ELISA.Immobilized mouse HVEM-Fch at 10 ?g/mL (100 ?l/well) can bind biotinylated mouse BTLA-Fc?The EC50 of biotinylated mouse BTLA-Fcis 64-96 ng/mL. Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Gln 206) of mouse HVEM (NP_849262.1) precursor was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus. Sequence: Met 1-Gln 206 Fusion Tag: C-His & Fc Accession: NP_849262.1 Species: Mouse Expressed Host: HEK293 Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: > 90 % as determined by SDS-PAGE Endotoxin: < 1.0 EU per ?g of the protein as determined by the LAL method Stability: Samples are stable for up to twelve months from date of receipt at -70?.Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Molecular Weight: 46.4 kDa Applied MM: 65 kDa Formulation: Lyophilized from sterile PBS, pH 7.4 Reconstitution: Please refer to the printed manual for detailed information. Background: Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy