Recombinant Mouse ASGPR1 / ASGR1 Protein (His tag)

Supplier: Elabscience Product Name: Recombinant Mouse ASGPR1 / ASGR1 Protein (His tag) Catalog No. PKSM040934 Product Type: recombinant protein Size: 100 ug Activity: Protein Construction: A DNA sequence encoding the extracellular domain (Ser 60-Asn 284) of mouse ASGPR1 (NP_033844.1) was expressed with a N-terminal polyhistidine tag. Sequence: Ser 60-Asn 284 Fusion Tag: N-His Accession: NP_033844.1 Species: Mouse Expressed Host: HEK293 Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: > 95 % as determined by SDS-PAGE Endotoxin: < 1.0 EU per ?g of the protein as determined by the LAL method Stability: Samples are stable for up to twelve months from date of receipt at -70?.Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Molecular Weight: 28 kDa Applied MM: 40-45 kDa Formulation: Lyophilized from sterile PBS, pH 7.4 Reconstitution: Please refer to the printed manual for detailed information. Background: The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.