Recombinant Mouse IL13RA2 / CD213A2 Protein (His tag)

Supplier: Elabscience Product Name: Recombinant Mouse IL13RA2 / CD213A2 Protein (His tag) Catalog No. PKSM040949 Product Type: recombinant protein Size: 100 ug Activity: Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140: 323. The ED50 for this effect is typically 0.1-0.5 ?g/mL. Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Lys 334) of mouse IL13R?2 (NP_032382.1) precursor was fused with a polyhistidine tag at the C-terminus. Sequence: Met 1-Lys 334 Fusion Tag: C-His Accession: NP_032382.1 Species: Mouse Expressed Host: HEK293 Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: > 98 % as determined by SDS-PAGE Endotoxin: < 1.0 EU per ?g of the protein as determined by the LAL method Stability: Samples are stable for up to twelve months from date of receipt at -70?.Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Molecular Weight: 38 kDa Applied MM: 45-50 kDa Formulation: Lyophilized from sterile PBS, pH 7.4 Reconstitution: Please refer to the printed manual for detailed information. Background: Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.