Figure 1: Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.
Mouse Monoclonal Antibody to GAPDH

Mouse Monoclonal Antibody to GAPDH

20035

Regular price $198.00 $168.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer’s and Huntington’s disease.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 1A10;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human GAPDH expressed in E. Coli. ;
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 37kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=65
Reference: 1. Allen R.W. J. Biol. Chem. 1987.262:649-653. ; 2. Sumner CJ. Ann Neurol 2003.54:6 47-54. ;