Mouse Monoclonal Antibody to TNF-alpha

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: TNF-alpha (tumor necrosis factor alpha) is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non covalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 2A9B9;
Isotype: Mouse IgG1
Immunogen: Recombinant Human TNF-alpha (BioSource company, Cat.No. PHC3013)
Formulation: Purified antibody in PBS containing 0.03% sodium azide.
MW: N/A
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=174
Reference: 1. Knowlton KU. Yajima T. J Am Coll Cardiol. 2004,Sep 15, 44(6):1298-300. ; 2. Reynolds JL. Ignatowski TA. Gallant S.et al. Brain Res. 2004,Oct 8, 1023(1):112-20. ; 3. Dulak J. Tomala K. Loboda A. et al. Life Sci. 2004,Oct 8, 75(21):2573-86. ;

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