Mouse Monoclonal Antibody to NT5E

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: 5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73). Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
Application: ELISA: 1/10000; IHC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 1D7;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of NT5E expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 70kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=346
Reference: 1. Oncol Rep. 2007 Jun;17(6):1341-6. ; 2. Neurochem Int. 2003 Dec;43(7):621-8. ;

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