Figure 1: Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Mouse Monoclonal Antibody to Rab25

Mouse Monoclonal Antibody to Rab25

20430

Regular price $308.00 $262.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Mouse
Clone: 3F12;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of Rab25 expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 24kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=396
Reference: 1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422 ; 2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146 ;