Mouse Monoclonal Antibody to Pirh2

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Rat
Clone: 1H10;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 30kDa; 60kDa (homodimer)
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=405
Reference: 1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50. ; 2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21. ; 3. Exp Cell Res. 2006 Oct 15;312(17):3370-8. ; 4. Cell. 2003 Mar 21;112(6):779-91.

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