Mouse Monoclonal Antibody to CDC2

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 8C5A7F10;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of CDC2 expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 34kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=440
Reference: 1. Mol Biol Cell. 2008 Aug;19(8):3536-43. ; 2. Eur J Cancer. 2009 May;45(8):1466-73.

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