Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell.
Mouse Monoclonal Antibody to CDC27

Mouse Monoclonal Antibody to CDC27

30064

Regular price $308.00 $262.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 5C12;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human CDC27 expressed in E. Coli. ;
Formulation: Ascitic fluid containing 0.03% sodium azide. ;
MW: 91kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=464
Reference: 1. Mol Cell Biol. 2004 May;24(9):3577-87. ; 2. J Biol Chem. 2004 Oct 1;279(40):42128-38.