Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Mouse Monoclonal Antibody to HK1

Mouse Monoclonal Antibody to HK1

30089

Regular price $308.00 $262.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Mouse; Rat;
Clone: 3A10;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human HK1 expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 102kDa
Storage: 4C; -20C for long term storage
Spplier link: http://www.promab.com/index.php?main_page=product_info&products_id=489
Reference: 1. Cell. 2005 Sep 23;122(6):957-68.; 2. Am J Hum Genet. 2006 Jan;78(1):78-88.