Mouse Monoclonal Antibody to EIF4E

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 5D11;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human EIF4E expressed in E. Coli. ;
Formulation: Ascitic fluid containing 0.03% sodium azide.
MW: 25kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=588
Reference: 1. Ann Surg Oncol. 2008 Nov;15(11):3207-15. ; 2. J Biol Chem. 2008 Sep 12;283(37):25227-37.

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