Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)

Mouse Monoclonal Antibody to NEDD8

30315

$341.00 You Pay

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 5B8;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human NEDD8 expressed in E. Coli. ;
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 9kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=714
Reference: 1. Cell. 2009 Jul 23;138(2):389-403. ; 2. Biochem Biophys Res Commun. 2009 Apr 10;381(3):443-7. ;

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