Mouse Monoclonal Antibody to AKT1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. ;
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: ; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Monkey; Rat
Clone: 1F7G2;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 55.7kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=4147
Reference: 1.Sci Rep. 2015 Jan 13;5:7758. ; 2.J Cancer Res Clin Oncol. 2015 Apr;141(4):615-26.;

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