Mouse Monoclonal Antibody to IDH1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.;
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: ; ICC: 1/50 - 1/250; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Mouse; Monkey
Clone: 7G8A1;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 21.3kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=4257
Reference: 1.Cancer Cell. 2015 Dec 14;28(6):773-84. ; 2.Int J Cancer. 2015 Sep 1;137(5):1058-65. ;

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