Adeno-Associated Virus Purification Mini Kit, 20 prepsV1169-02
Adeno-associated viruses, belong to the replication deficient
parvovirus family, are small single-stranded DNA viruses. AAVs are important
gene delivery tools, which have been used in gene therapy and RNAi delivery.
Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl
ultracentrifugation procedure is time consuming and limited to the volume of
cell lysate to be processed. The ViraTrapTM AAV mini purification kit is designed
for efficient purification of recombinant AAV2 and AAVDJ from rAAV vector
transfected cell line. Up to 1 x 1012 viral particles can be purified
from cell lysate of 2 x T75 flasks. Each column can be regenerated for purifying
the same rAAV. For optimized viral binding and recovery, each column can be
re-generated only once.
Familiar with each step by reading this menu and prepare all materials for the procedure.
The guaranteed shelf life is 12 months from the date of purchase.
The adenovirus infected cell media and the purified virus can
be potential bio-hazardous material and can be infectious to human and animals.
All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials Required But Not Supplied:
0.45 μm filter unit and 0.22 μm syringe filter
Rack holder for column