AFAP1 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.
Research Areas: Signal transduction
Applications: WB,IHC,ELISA
Recommended Dilutions: WB 1:200-1:1000, IHC 1:25-1:100
Size: 200 uL
Concentration: 0.1mg/mL
Swissprot: Q8N556
Gene Accession: BC032777
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant protein of human AFAP1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 81kDa
Tissue Specificity/Positive Control: Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Cellular Localization: Cytoplasm>cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-afap1_polyclonal_antibody-30791.html
Manual Link: https://www.elabscience.com/viewpdf-30791-elabscience-E-AB-10747.PDF