Product Type: Antibody
Descritpion: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Research Areas: Cancer, Cell Biology, Cardiovascular, Metabolism, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:50-1:200
Size: 200 uL
Gene Accession: BC002699
Immunogen: Recombinant protein of human ATG5
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 32kDa
Tissue Specificity/Positive Control: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Cellular Localization: Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-atg5_polyclonal_antibody-30858.html
Manual Link: https://www.elabscience.com/viewpdf-30858-elabscience-E-AB-10814.PDF