RASSF1 Polyclonal AntibodyE-AB-11524
Product Type: Antibody
Descritpion: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal transduction
Recommended Dilutions: IHC 1:20-1:100
Size: 200 uL
Gene Accession: BC110412
Immunogen: Recombinant protein of human RASSF1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Tissue Specificity/Positive Control: Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
Cellular Localization: Nucleus. Predominantly nuclear and Cytoplasm>cytoskeleton. Cytoplasm>cytoskeleton>centrosome. Cytoplasm>cytoskeleton>spindle. Cytoplasm>cytoskeleton>spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-rassf1_polyclonal_antibody-31564.html
Manual Link: https://www.elabscience.com/viewpdf-31564-elabscience-E-AB-11524.PDF