WT1 Polyclonal AntibodyE-AB-11741
Product Type: Antibody
Descritpion: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Developmental biology, Tags & Cell Markers
Recommended Dilutions: WB 1:500-1:2000
Size: 200 uL
Immunogen: Recombinant protein of human WT1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 49kDa
Tissue Specificity/Positive Control: Expressed in the kidney and a subset of hematopoietic cells.
Cellular Localization: Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus>nucleoplasm and Nucleus speckle.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-wt1_polyclonal_antibody-39760.html
Manual Link: https://www.elabscience.com/viewpdf-39760-elabscience-E-AB-11741.PDF