Product Type: Antibody
Descritpion: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Research Areas: Cancer, Signal transduction
Recommended Dilutions: WB 1:500-1:1000, IF/ICC 1:100-1:200
Size: 200 uL
Immunogen: Synthetic peptide of human VASP (Ab-157)
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 40kDa
Tissue Specificity/Positive Control: Highly expressed in platelets.
Cellular Localization: Cytoplasm. Cytoplasm>cytoskeleton. Cell junction>focal adhesion. Cell projection>lamellipodium membrane. Cell projection>filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-vasp_polyclonal_antibody-39935.html
Manual Link: https://www.elabscience.com/viewpdf-39935-elabscience-E-AB-12094.PDF