Product Type: Antibody
Descritpion: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:1000, IHC 1:50-100, IF/ICC 1:100-1:200
Size: 200 uL
Immunogen: Synthetic peptide of human H2AFX (Ab-139)
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 15kDa
Tissue Specificity/Positive Control:
Cellular Localization: Nucleus. Chromosome.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-histone_h2a.x_polyclonal_antibody-39945.html
Manual Link: https://www.elabscience.com/viewpdf-39945-elabscience-E-AB-12104.PDF