SMAD2 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal transduction, Stem cells
Applications: WB,IHC
Recommended Dilutions: WB 1:500-1:2000, IHC 1:50-100
Size: 200 uL
Concentration: 1mg/mL
Swissprot: Q15796
Gene Accession:
Reactivity: Human
,Mouse
,Rat

Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide of human SMAD2 (Ab-220)
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 52kDa
Tissue Specificity/Positive Control: Expressed at high levels in skeletal muscle, heart and placenta.
Cellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-smad2_polyclonal_antibody-39952.html
Manual Link: https://www.elabscience.com/viewpdf-39952-elabscience-E-AB-12111.PDF