WWTR1 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Stem cells
Applications: WB
Recommended Dilutions: WB 1:500-1:1000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: Q9GZV5
Gene Accession:
Reactivity: Human
,Mouse
,Rat

Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide of human WWTR1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 44kDa
Tissue Specificity/Positive Control: Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
Cellular Localization: Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-wwtr1_polyclonal_antibody-40002.html
Manual Link: https://www.elabscience.com/viewpdf-40002-elabscience-E-AB-12161.PDF