AKT1 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Applications: WB,ELISA
Recommended Dilutions: WB 1:500-1:1000
Size: 200 uL
Concentration: 0.1mg/mL
Swissprot: P31749
Gene Accession: NP_005154
Reactivity: Human
,Mouse
,Rat

Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide of human pan-AKT
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 56kDa
Tissue Specificity/Positive Control: Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Cellular Localization: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-akt1_polyclonal_antibody-40053.html
Manual Link: https://www.elabscience.com/viewpdf-40053-elabscience-E-AB-12213.PDF