PLEKHO1 Polyclonal AntibodyE-AB-12325
Product Type: Antibody
Descritpion: Pleckstrin homology domain-containing family O member 1 is a protein that in humans is encoded by the PLEKHO1 gene.PLEKHO1 has been shown to interact with Casein kinase 2, alpha 1 and SMURF1.It plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). Appears to target ATM to the plasma membrane,and also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. The protein implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
Research Areas: Signal transduction
Recommended Dilutions: IHC 1:25-1:100
Size: 200 uL
Gene Accession: NP_057358.2
Immunogen: Synthetic peptide of human PLEKHO1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Tissue Specificity/Positive Control: Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cancer cell lines such as MOLT-4, HEK293 and Jurkat cells.
Cellular Localization: Cell membrane. Nucleus. Cytoplasm. Predominantly localized to the plasma membrane. In C2C12 cells, with the absence of growth factor, it is found in the nucleus. It rapidly translocates to the plasma membrane after insulin stimulation. In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3. However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-plekho1_polyclonal_antibody-31780.html
Manual Link: https://www.elabscience.com/viewpdf-31780-elabscience-E-AB-12325.PDF