Product Type: Antibody
Descritpion: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes whicHuman, Mouseediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.
Research Areas: Cancer, Cell Biology
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:500
Size: 200 uL
Gene Accession: NP_001008895
Immunogen: Synthetic peptide of human CUL4A
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 88kDa
Tissue Specificity/Positive Control:
Cellular Localization: Nucleus,nucleoplasm,Other locations:Cul4-RING E3 ubiquitin ligase complex,Cul4A-RING E3 ubiquitin ligase complex
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-cul4a_polyclonal_antibody-31794.html
Manual Link: https://www.elabscience.com/viewpdf-31794-elabscience-E-AB-12340.PDF