DAP5 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Applications: WB,IHC,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300
Size: 200 uL
Concentration: 0.5mg/mL
Swissprot: P78344
Gene Accession: NP_001409
Reactivity: Human,Mouse
Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide of human EIF4G2
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 103kDa
Tissue Specificity/Positive Control: Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Cellular Localization: Cytosol,Other locations:cell-cell adherens junction,eukaryotic translation initiation factor 4F complex,membrane
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-dap5_polyclonal_antibody-31820.html
Manual Link: https://www.elabscience.com/viewpdf-31820-elabscience-E-AB-12366.PDF