Product Type: Antibody
Descritpion: Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags & Cell Markers
Recommended Dilutions: WB 1:200-1:1000, IHC 1:15-1:50
Size: 200 uL
Gene Accession: NP_001076044
Immunogen: Synthetic peptide of human RBFOX3
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 34kDa
Tissue Specificity/Positive Control:
Cellular Localization: Nucleus. Cytoplasm.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-rbfox3_polyclonal_antibody-40065.html
Manual Link: https://www.elabscience.com/viewpdf-40065-elabscience-E-AB-12595.PDF