E-Cadherin Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
Research Areas: Cancer, Developmental biology, Signal transduction
Applications: IHC,ELISA
Recommended Dilutions: IHC 1:25-1:100
Size: 200 uL
Concentration: 0.2mg/mL
Swissprot: P12830
Gene Accession: NP_004351
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide of human pan CDH
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW:
Tissue Specificity/Positive Control: Non-neural epithelial tissues.
Cellular Localization: Cell junction. Cell membrane. Endosome. Golgi apparatus>trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-e_cadherin_polyclonal_antibody-32372.html
Manual Link: https://www.elabscience.com/viewpdf-32372-elabscience-E-AB-12930.PDF