LIN28B Polyclonal AntibodyE-AB-13008
Product Type: Antibody
Descritpion: The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:2000
Size: 200 uL
Gene Accession: NP_001004317
Immunogen: Synthetic peptide of human LIN28B
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 27kDa
Tissue Specificity/Positive Control: High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
Cellular Localization: Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-lin28b_polyclonal_antibody-32450.html
Manual Link: https://www.elabscience.com/viewpdf-32450-elabscience-E-AB-13008.PDF