Product Type: Antibody
Descritpion: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction, Stem cells
Recommended Dilutions: WB 1:500-1:2000, IHC 1:25-1:100
Size: 200 uL
Gene Accession: NP_005893
Immunogen: Synthetic peptide of human SMAD3
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 48kDa
Tissue Specificity/Positive Control:
Cellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-smad3_polyclonal_antibody-32506.html
Manual Link: https://www.elabscience.com/viewpdf-32506-elabscience-E-AB-13066.PDF