Product Type: Antibody
Descritpion: Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. Bod1-depleted cells form syntelic attachments that can oscillate and generate enough force to separate sister kinetochores, suggesting that microtubule-kinetochore interactions were intact. Releasing Bod1-depleted cells from a monastrol block increases the frequency of syntelic attachments and the number of cells displaying biorientation defects.
Research Areas: Cell Biology, Epigenetics and Nuclear Signaling
Recommended Dilutions: IHC 1:25-1:100
Size: 200 uL
Gene Accession: NP_001153123
Immunogen: Synthetic peptide of human BOD1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Tissue Specificity/Positive Control:
Cellular Localization: Cytoplasm>cytoskeleton>centrosome. Chromosome>centromere>kinetochore. Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-bod1_polyclonal_antibody-32535.html
Manual Link: https://www.elabscience.com/viewpdf-32535-elabscience-E-AB-13095.PDF