Product Type: Antibody
Descritpion: The deduced full-length ACAD11 protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable ACAD11 expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11 associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.
Research Areas: Cancer, Metabolism, Signal transduction
Recommended Dilutions: WB 1:200-1:1000, IHC 1:15-1:50
Size: 200 uL
Gene Accession: BC125204
Immunogen: Recombinant protein of human ACAD11
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 87kDa
Tissue Specificity/Positive Control: Widely expressed with highest levels in brain followed by liver, heart and kidney.
Cellular Localization: Peroxisome.
Storage: Store at -20C. Avoid freeze / thaw cycles.