ANLN Polyclonal AntibodyE-AB-14567
Product Type: Antibody
Descritpion: ?Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis.
Research Areas: Cancer, Cell Biology, Signal transduction
Recommended Dilutions: IHC 1:25-1:100
Size: 200 uL
Gene Accession: BC034692
Immunogen: Recombinant protein of human ANLN
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Tissue Specificity/Positive Control: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Cellular Localization: Nucleus. Cytoplasm>cytoskeleton. Cytoplasm>cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-anln_polyclonal_antibody-33995.html
Manual Link: https://www.elabscience.com/viewpdf-33995-elabscience-E-AB-14567.PDF