Product Type: Antibody
Descritpion: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:50-1:200
Size: 200 uL
Gene Accession: BC001810
Immunogen: Recombinant protein of human ENO1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 47kDa
Tissue Specificity/Positive Control: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Cellular Localization: Nucleus and Cytoplasm. Cell membrane. Cytoplasm>myofibril>sarcomere>M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Storage: Store at -20C. Avoid freeze / thaw cycles.