BUB1 Polyclonal Antibody
BUB1 Polyclonal Antibody

BUB1 Polyclonal Antibody

E-AB-14807

Regular price $399.00 $349.00 You Pay

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Research Areas: Cancer, Cell Biology
Applications: IHC,ELISA
Recommended Dilutions: IHC 1:50-1:200
Size: 200 uL
Concentration: 0.2mg/mL
Swissprot: O43683
Gene Accession: BC028201
Reactivity: Human
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant protein of human BUB1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW:
Tissue Specificity/Positive Control: High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
Cellular Localization: Nucleus. Chromosome>centromere>kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-bub1_polyclonal_antibody-34235.html
Manual Link: https://www.elabscience.com/viewpdf-34235-elabscience-E-AB-14807.PDF