Product Type: Antibody
Descritpion: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Research Areas: Cancer, Metabolism, Signal transduction
Recommended Dilutions: WB 1:500-2000, IHC 1:30-150, ELISA 1:2000-5000
Size: 200 uL
Gene Accession: BC039084
Immunogen: Fusion protein of human DLAT
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 69 kDa
Tissue Specificity/Positive Control:
Cellular Localization: Mitochondrion matrix.
Storage: Store at -20C. Avoid freeze / thaw cycles.